Hotspot

trnL-CAA-ycf2

Genome · 86686-87194 · 509 bp

Back to Melilotus

Pi 0.492

MarkerSeek score 79.00

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: Genome
length_bp: 509
variable_sites: 213
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	AGGGTGAGCTTCAAGAGACT	50.0	50.0	58.0	58.0	1093 bp	0.5	59.8
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	AGGGTGAGCTTCAAGAGACT	50.0	50.0	58.0	58.0	1095 bp	0.5	59.7
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	TTCGAAGAAGGGGAAGGAGT	50.0	50.0	58.0	58.0	1292 bp	0.5	59.7
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	TTCGAAGAAGGGGAAGGAGT	50.0	50.0	58.0	58.0	1294 bp	0.5	59.7
trnL-CAA-ycf2_p5	CTCGGGATGACATGACGATC	AGGGTGAGCTTCAAGAGACT	55.0	50.0	57.9	58.0	1218 bp	0.5	59.4