Hotspot

rpl23-trnL-CAA

Genome · 86287-86604 · 318 bp

Back to Melilotus

Pi 0.19

MarkerSeek score 76.80

Primer available yes

Label rpl23-trnL-CAA

Feature Metadata

label_name: rpl23-trnL-CAA
feature_type: igs
region: Genome
length_bp: 318
variable_sites: 58
pi: 0.19
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnL-CAA_p1	AAACCGCACTCAAAGGTAGG	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.1	58.0	1052 bp	0.5	59.1
rpl23-trnL-CAA_p2	AAACCGCACTCAAAGGTAGG	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.1	58.0	1050 bp	0.5	59.1
rpl23-trnL-CAA_p3	AAACCGCACTCAAAGGTAGG	GTGATCTGCTGGCGAACTTA	50.0	50.0	58.1	58.0	1150 bp	0.5	59.1
rpl23-trnL-CAA_p4	CCCTGCGGTAATGATTCCTC	GTCTCGCGTGTCTACCATTT	55.0	50.0	58.1	58.0	802 bp	0.5	59.0
rpl23-trnL-CAA_p5	CCCTGCGGTAATGATTCCTC	CTCGCGTGTCTACCATTTCA	55.0	50.0	58.1	58.0	800 bp	0.5	59.0