Hotspot

psbA

LSC · 431-1492 · 1062 bp

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Pi 0.00847

MarkerSeek score 49.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 9
pi: 0.00847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CTGCCTTAATCCACTTGGCT	ACATAGGGAAAGCTGTGTGC	50.0	50.0	57.9	58.2	1821-1846 bp	1.0	79.4
psbA_p2	CTGCCTTAATCCACTTGGCT	CATAGGGAAAGCTGTGTGCA	50.0	50.0	57.9	58.2	1820-1845 bp	1.0	79.4
psbA_p3	CTGCCTTAATCCACTTGGCT	TATGCTTGGGAGTCCCTGAT	50.0	50.0	57.9	57.8	1494-1511 bp	1.0	79.4
psbA_p4	ACTGCCTTAATCCACTTGGC	ACATAGGGAAAGCTGTGTGC	50.0	50.0	58.2	58.2	1822-1847 bp	1.0	79.2
psbA_p5	ACTGCCTTAATCCACTTGGC	CATAGGGAAAGCTGTGTGCA	50.0	50.0	58.2	58.2	1821-1846 bp	1.0	79.1