Hotspot

petB

LSC · 77015-78409 · 1395 bp

Back to Melicope

Pi 0.00717

MarkerSeek score 62.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1395
variable_sites: 10
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTGATTGGTAGTTCGACCGC	AGGAGTGATTACAAATCGCT	50.0	40.0	58.3	54.5	1487-1490 bp	1.0	50.0
petB_p2	TTGATTGGTAGTTCGACCGC	AAGGAGTGATTACAAATCGCT	50.0	38.1	58.3	55.3	1488-1491 bp	1.0	48.7
petB_p3	TTGATTGGTAGTTCGACCGC	AAAGGAGTGATTACAAATCGCT	50.0	36.4	58.3	56.1	1489-1492 bp	1.0	46.9
petB_p4	TTGATTGGTAGTTCGACCGC	AAGGAGTGATTACAAATCGC	50.0	40.0	58.3	53.7	1488-1491 bp	1.0	43.9
petB_p5	AGTTGATTGGTAGTTCGACCG	AGGAGTGATTACAAATCGCT	47.6	40.0	58.0	54.5	1489-1492 bp	1.0	43.9