Hotspot

psaJ-rpl33

LSC · 69198-69679 · 482 bp

Back to Melicope

Pi 0.00887

MarkerSeek score 63.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 482
variable_sites: 4
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGGGTCTTTAGCAGGGTTA	GGACATCTTTACCCTTGGCC	50.0	55.0	58.1	58.2	553-584 bp	1.0	82.2
psaJ-rpl33_p2	TCGGGTCTTTAGCAGGGTTA	TTGCTCGGACATCTTTACCC	50.0	50.0	58.1	57.6	559-590 bp	1.0	80.9
psaJ-rpl33_p3	TCGGGTCTTTAGCAGGGTTA	ACCCTTTCGAACACAACGAG	50.0	50.0	58.1	58.4	597-628 bp	1.0	80.7
psaJ-rpl33_p4	TCGGGTCTTTAGCAGGGTTA	CCCTTTCGAACACAACGAGT	50.0	50.0	58.1	58.4	596-627 bp	1.0	80.7
psaJ-rpl33_p5	TCGGGTCTTTAGCAGGGTTA	TGCTCGGACATCTTTACCCT	50.0	50.0	58.1	58.4	558-589 bp	1.0	80.6