Hotspot

psaA-ycf3

LSC · 43677-44410 · 734 bp

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Pi 0.00681

MarkerSeek score 62.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 5
pi: 0.00681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	854 bp	1.0	78.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	846 bp	1.0	78.5
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCTATTGCGCTTACTCCTG	50.0	50.0	57.9	57.8	887 bp	1.0	77.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCAAGCGCAGAATTGGTTGA	50.0	45.0	57.9	58.4	857 bp	1.0	76.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCGCAGAATTGGTTGAA	50.0	45.0	57.9	57.6	856 bp	1.0	76.2