Hotspot

rpoB-trnC-GCA

LSC · 27400-28615 · 1216 bp

Back to Melicope

Pi 0.0111

MarkerSeek score 63.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 13
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCGCATTTCCATCTCC	CCAGATTTGAACCGGGGAAA	50.0	50.0	58.0	58.1	1267-1304 bp	1.0	83.1
rpoB-trnC-GCA_p2	ATTCCCGCATTTCCATCTCC	ATCGTTTGTTGATCAGGCGA	50.0	45.0	58.0	57.9	1290-1327 bp	1.0	82.9
rpoB-trnC-GCA_p3	ATTCCCGCATTTCCATCTCC	CGAGCGGAATAGGGGATTTC	50.0	55.0	58.0	58.2	1317-1354 bp	1.0	82.0
rpoB-trnC-GCA_p4	ATTCCCGCATTTCCATCTCC	GTTTGTTGATCAGGCGACAC	50.0	50.0	58.0	57.7	1287-1324 bp	1.0	81.2
rpoB-trnC-GCA_p5	ATTCCCGCATTTCCATCTCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1249-1286 bp	1.0	81.0