Hotspot

atpF-atpH

LSC · 13329-13794 · 466 bp

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Pi 0.0108

MarkerSeek score 63.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 5
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCAAAACTCCCGGC	GGACTGGTTGTAGCCTTAGC	50.0	55.0	58.7	58.0	582-583 bp	1.0	75.8
atpF-atpH_p2	TTAAACCCAAAACTCCCGGC	GACTGGTTGTAGCCTTAGCG	50.0	55.0	58.7	58.4	581-582 bp	1.0	73.2
atpF-atpH_p3	TAAACCCAAAACTCCCGGC	GGACTGGTTGTAGCCTTAGC	52.6	55.0	58.0	58.0	581-582 bp	1.0	72.9
atpF-atpH_p4	GGCCAGTGAATCAAAGAAACG	GGACTGGTTGTAGCCTTAGC	47.6	55.0	58.1	58.0	558-559 bp	1.0	72.7
atpF-atpH_p5	CGAATGGCCAGTGAATCAAAG	GGACTGGTTGTAGCCTTAGC	47.6	55.0	57.9	58.0	563-564 bp	1.0	72.1