Hotspot

trnS-GCU-trnG-UCC

LSC · 8195-9189 · 995 bp

Back to Melicope

Pi 0.0145

MarkerSeek score 56.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 14
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	1033-1046 bp	1.0	51.1
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	1034-1047 bp	1.0	50.0
trnS-GCU-trnG-UCC_p3	ACGCTTTAATCCACTCAGCC	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1030-1043 bp	1.0	49.1
trnS-GCU-trnG-UCC_p4	CGCTTTAATCCACTCAGCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1029-1042 bp	1.0	49.1
trnS-GCU-trnG-UCC_p5	TCCGACGCTTTAATCCACTC	TACCACTAAACTATACCCGC	50.0	45.0	57.7	54.0	1034-1047 bp	1.0	48.8