Hotspot

trnK-UUU-rps16

LSC · 4354-4899 · 546 bp

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Pi 0.0307

MarkerSeek score 68.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 16
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	651-652 bp	1.0	84.9
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	640-641 bp	1.0	83.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	639-640 bp	1.0	83.7
trnK-UUU-rps16_p4	AGCCGTACTTAAAAGCCGAG	TCAAAGAAAGCGGGGGTTTT	50.0	45.0	58.0	58.2	648-649 bp	1.0	83.3
trnK-UUU-rps16_p5	AGCCGTACTTAAAAGCCGAG	TTCAAAGAAAGCGGGGGTTT	50.0	45.0	58.0	58.2	649-650 bp	1.0	83.3