Hotspot

trnH-GUG-psbA

LSC · 72-430 · 359 bp

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Pi 0.0407

MarkerSeek score 74.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 14
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	484-501 bp	1.0	78.2
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCGTGCTAACCTTGGTATGG	50.0	50.0	57.9	57.3	485-502 bp	1.0	78.2
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CGTGCTAACCTTGGTATGGA	50.0	50.0	58.2	57.3	485-502 bp	1.0	77.9
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TCGTGCTAACCTTGGTATGG	50.0	50.0	58.2	57.3	486-503 bp	1.0	77.9
trnH-GUG-psbA_p5	CAACCCACTGCCTTAATCCA	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.8	57.3	491-508 bp	1.0	77.5