Hotspot

rpl16

LSC · 77604-79053 · 1450 bp

Back to Melica

Pi 0.00699
MarkerSeek score 61.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1450
variable_sites
110
pi
0.00699
unique_haplotype_count
51
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GAACGAAAAACCTGGCGTTT TTCCCGAAATCCCTGAAAAA 45.0 40.0 57.8 55.1 1431-1586 bp 0.95 63.3
rpl16_p2 GAACGAAAAACCTGGCGTTT TTTCCCGAAATCCCTGAAAA 45.0 40.0 57.8 55.1 1432-1587 bp 0.95 63.3
rpl16_p3 GAACGAAAAACCTGGCGTTT TTTTCCCGAAATCCCTGAAA 45.0 40.0 57.8 55.1 1433-1588 bp 0.95 63.3
rpl16_p4 GAACGAAAAACCTGGCGTTT TTTTTCCCGAAATCCCTGAA 45.0 40.0 57.8 55.1 1434-1589 bp 0.95 63.3
rpl16_p5 GAACGAAAAACCTGGCGTTT TTTCCCGAAATCCCTGAAAAA 45.0 38.1 57.8 56.0 1432-1587 bp 0.95 61.9