Hotspot

ndhF-trnL

SSC · 103591-105281 · 1691 bp

Back to Melica

Pi 0.0131
MarkerSeek score 78.10
Primer available yes
Label ndhF-trnL

Feature Metadata

label_name
ndhF-trnL
feature_type
igs
region
SSC
length_bp
1691
variable_sites
203
pi
0.0131
unique_haplotype_count
56
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL_p1 GGAAGTGGGAGAAGAGGGAT TTCGAACCGAGATGCTTGAG 55.0 50.0 58.1 58.0 1421-1844 bp 0.983333 90.4
ndhF-trnL_p2 GGAAGTGGGAGAAGAGGGAT GGATTCGAACCGAGATGCTT 55.0 50.0 58.1 58.1 1424-1847 bp 0.983333 90.0
ndhF-trnL_p3 GGAAGTGGGAGAAGAGGGAT TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 1394-1817 bp 0.983333 89.4
ndhF-trnL_p4 GGAAGTGGGAGAAGAGGGAT AGCAGCGTGTCTACCAATTT 55.0 45.0 58.1 57.8 1387-1810 bp 0.983333 88.9
ndhF-trnL_p5 GGAAGTGGGAGAAGAGGGAT GCTTGAGCACTGCTTCCTAA 55.0 50.0 58.1 58.2 1408-1831 bp 0.983333 88.9