Hotspot

petA-psbJ

LSC · 60501-61325 · 825 bp

Back to Melica

Pi 0.00946
MarkerSeek score 62.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
825
variable_sites
77
pi
0.00946
unique_haplotype_count
49
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCCGTTATTTTGGCACA TCTGGATTGGGTTCATCTCT 45.0 45.0 57.4 55.5 851-957 bp 1.0 65.8
petA-psbJ_p2 GATCCATTACGCGTCCAAGG TCTGGATTGGGTTCATCTCT 55.0 45.0 58.8 55.5 887-993 bp 1.0 64.6
petA-psbJ_p3 TGCATCCGTTATTTTGGCAC TCTGGATTGGGTTCATCTCT 45.0 45.0 57.4 55.5 852-958 bp 0.966667 64.4
petA-psbJ_p4 GCATCCGTTATTTTGGCACA TTCTGGATTGGGTTCATCTCT 45.0 42.9 57.4 56.3 852-958 bp 1.0 64.3
petA-psbJ_p5 TGCATCCGTTATTTTGGCAC TTCTGGATTGGGTTCATCTCT 45.0 42.9 57.4 56.3 853-959 bp 0.966667 62.9