Hotspot

trnT-tRNA-Leu

LSC · 46437-47202 · 766 bp

Back to Melica

Pi 0.00746
MarkerSeek score 73.20
Primer available yes
Label trnT-tRNA-Leu

Feature Metadata

label_name
trnT-tRNA-Leu
feature_type
igs
region
LSC
length_bp
766
variable_sites
80
pi
0.00746
unique_haplotype_count
45
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-tRNA-Leu_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 799-927 bp 1.0 91.2
trnT-tRNA-Leu_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 791-919 bp 1.0 91.0
trnT-tRNA-Leu_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 769-897 bp 1.0 90.6
trnT-tRNA-Leu_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 789-917 bp 1.0 89.4
trnT-tRNA-Leu_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 798-926 bp 1.0 89.1