Hotspot

psbM-trnC

LSC · 17189-18893 · 1705 bp

Back to Melica

Pi 0.00856
MarkerSeek score 74.00
Primer available yes
Label psbM-trnC

Feature Metadata

label_name
psbM-trnC
feature_type
igs
region
LSC
length_bp
1705
variable_sites
158
pi
0.00856
unique_haplotype_count
53
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnC_p1 TGCTACTGCACTGTTCATTCT AGTTCAAATCTGGGTGCCG 42.9 52.6 57.6 58.1 1399-1817 bp 1.0 71.5
psbM-trnC_p2 TGCTACTGCACTGTTCATTCT CCAGTTCAAATCTGGGTGCC 42.9 55.0 57.6 59.1 1401-1819 bp 1.0 71.0
psbM-trnC_p3 TGCTACTGCACTGTTCATTCT CAGTTCAAATCTGGGTGCCG 42.9 55.0 57.6 59.5 1400-1818 bp 1.0 68.1
psbM-trnC_p4 TGCTACTGCACTGTTCATTC AGTTCAAATCTGGGTGCCG 45.0 52.6 56.1 58.1 1399-1817 bp 1.0 67.3
psbM-trnC_p5 TGCTACTGCACTGTTCATTCT AGGGGACTGCAAATCCTTTA 42.9 45.0 57.6 56.4 1424-1842 bp 1.0 67.0