Hotspot

ycf1

SSC · 128962-134427 · 5466 bp

Back to Melia

Pi 0.0135
MarkerSeek score 45.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5466
variable_sites
74
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CCATACTGAAACGACTGCCA CAGGCCAAGAGAATCCGAAA 50.0 50.0 57.9 57.9 894 bp 1.0 79.8
ycf1_p2 ACCATACTGAAACGACTGCC CAGGCCAAGAGAATCCGAAA 50.0 50.0 57.9 57.9 895 bp 1.0 79.8
ycf1_p3 CCATACTGAAACGACTGCCA TCGTGCTACTTTGGAATGCA 50.0 45.0 57.9 57.8 1086 bp 1.0 79.2
ycf1_p4 CCATACTGAAACGACTGCCA ATCCGGGATTGATTGGAGGA 50.0 50.0 57.9 58.2 1169 bp 1.0 79.2
ycf1_p5 ACCATACTGAAACGACTGCC TCGTGCTACTTTGGAATGCA 50.0 45.0 57.9 57.8 1087 bp 1.0 79.1