Hotspot

rpl16

LSC · 85092-86534 · 1443 bp

Back to Melia

Pi 0.0112
MarkerSeek score 52.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1443
variable_sites
16
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CGCACCAAAAACTCGAGTTC ATACTCCGGTGTTGAGGGAA 50.0 50.0 58.0 58.1 2518-2563 bp 1.0 80.7
rpl16_p2 CGCACCAAAAACTCGAGTTC TACTCCGGTGTTGAGGGAAT 50.0 50.0 58.0 58.1 2517-2562 bp 1.0 80.7
rpl16_p3 ACACATTAATTCTCGGGCCC ATACTCCGGTGTTGAGGGAA 50.0 50.0 57.9 58.1 2278-2323 bp 1.0 80.5
rpl16_p4 ACACATTAATTCTCGGGCCC TACTCCGGTGTTGAGGGAAT 50.0 50.0 57.9 58.1 2277-2322 bp 1.0 80.5
rpl16_p5 GGCGATTACTGGCTCCTATG TACTCCGGTGTTGAGGGAAT 55.0 50.0 57.9 58.1 2305-2350 bp 1.0 80.2