Hotspot

petB

LSC · 78691-80161 · 1471 bp

Back to Melia

Pi 0.00884
MarkerSeek score 55.70
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1471
variable_sites
13
pi
0.00884
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TTGATTGGTAGTTCGACCGC CCTCCCCCAAGCAAAAAGAT 50.0 50.0 58.3 58.1 1589-1618 bp 1.0 78.1
petB_p2 TTGATTGGTAGTTCGACCGC TTCCTCCCCCAAGCAAAAAG 50.0 50.0 58.3 58.3 1591-1620 bp 1.0 76.3
petB_p3 TTGATTGGTAGTTCGACCGC TTATTGTTCCTCCCCCAAGC 50.0 50.0 58.3 57.5 1597-1626 bp 1.0 74.3
petB_p4 TTGATTGGTAGTTCGACCGC GTTCCTCCCCCAAGCAAAAA 50.0 50.0 58.3 58.6 1592-1621 bp 1.0 73.9
petB_p5 TTGATTGGTAGTTCGACCGC TATTGTTCCTCCCCCAAGCA 50.0 50.0 58.3 58.6 1596-1625 bp 1.0 73.6