Hotspot

rpl32-trnL-UAG

SSC · 118048-119166 · 1119 bp

Back to Melia

Pi 0.0171
MarkerSeek score 57.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1119
variable_sites
19
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCGGGAATTCAAAAAGTTT AGCGTGTCTACCGATTTCAC 35.0 50.0 53.9 58.0 1190-1233 bp 1.0 48.5
rpl32-trnL-UAG_p2 ACCGGGAATTCAAAAAGTTT CAGCGTGTCTACCGATTTCA 35.0 50.0 53.9 58.0 1191-1234 bp 1.0 48.5
rpl32-trnL-UAG_p3 ACCGGGAATTCAAAAAGTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.9 57.9 1200-1243 bp 1.0 47.5
rpl32-trnL-UAG_p4 ACCGGGAATTCAAAAAGTTTT AGCGTGTCTACCGATTTCAC 33.3 50.0 54.7 58.0 1190-1233 bp 1.0 47.5
rpl32-trnL-UAG_p5 ACCGGGAATTCAAAAAGTTTT CAGCGTGTCTACCGATTTCA 33.3 50.0 54.7 58.0 1191-1234 bp 1.0 47.5