Hotspot

ndhF-rpl32

SSC · 116850-117888 · 1039 bp

Back to Melia

Pi 0.0215
MarkerSeek score 67.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1039
variable_sites
21
pi
0.0215
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT GCTGCCCAATACCCTTTCTT 50.0 50.0 57.7 58.1 1115-1174 bp 1.0 82.2
ndhF-rpl32_p2 CTGGAAGTGGAACGAAAGGT AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.7 57.8 1128-1187 bp 1.0 82.0
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT ATGCTGCCCAATACCCTTTC 50.0 50.0 57.7 58.2 1117-1176 bp 1.0 81.7
ndhF-rpl32_p4 CTGGAAGTGGAACGAAAGGT AATGCTGCCCAATACCCTTT 50.0 45.0 57.7 57.7 1118-1177 bp 1.0 81.4
ndhF-rpl32_p5 CTGGAAGTGGAACGAAAGGT CAATGCTGCCCAATACCCTT 50.0 50.0 57.7 58.5 1119-1178 bp 1.0 79.3