Hotspot

psbT-psbN

LSC · 78054-78118 · 65 bp

Back to Melia

Pi 0.0615
MarkerSeek score 82.70
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
65
variable_sites
4
pi
0.0615
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 CGAGAACCACCTAAAGTTCCA GATCCATTCGAGGAACACGG 47.6 55.0 57.6 58.4 125 bp 1.0 68.0
psbT-psbN_p2 CGAGAACCACCTAAAGTTCCA CATTCGAGGAACACGGAGAT 47.6 50.0 57.6 57.4 121 bp 1.0 66.8
psbT-psbN_p3 CGAGAACCACCTAAAGTTCCA AGATCCATTCGAGGAACACG 47.6 50.0 57.6 57.4 126 bp 1.0 66.7
psbT-psbN_p4 CGAGAACCACCTAAAGTTCCA GGCAACCCTCTCAACAACTA 47.6 50.0 57.6 57.4 148 bp 1.0 66.5
psbT-psbN_p5 TCGAGAACCACCTAAAGTTCC GATCCATTCGAGGAACACGG 47.6 55.0 57.3 58.4 126 bp 1.0 65.7