Hotspot

ycf3-trnS-GGA

LSC · 47505-48383 · 879 bp

Back to Melia

Pi 0.0122
MarkerSeek score 65.00
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
879
variable_sites
10
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CACTTATCCGCGATCTAGGC ACCCCCGGTAAACAAAAGTC 55.0 50.0 58.0 58.0 909-969 bp 1.0 84.6
ycf3-trnS-GGA_p2 TCTCCTGAAGTGGTCGGAAT ACCCCCGGTAAACAAAAGTC 50.0 50.0 58.1 58.0 982-1042 bp 1.0 84.2
ycf3-trnS-GGA_p3 CACTTATCCGCGATCTAGGC TGCTACGCCTTAAACCACTC 55.0 50.0 58.0 57.9 873-933 bp 1.0 84.0
ycf3-trnS-GGA_p4 TCTCCTGAAGTGGTCGGAAT TGCTACGCCTTAAACCACTC 50.0 50.0 58.1 57.9 946-1006 bp 1.0 83.6
ycf3-trnS-GGA_p5 CACTTATCCGCGATCTAGGC CCCCCGGTAAACAAAAGTCT 55.0 50.0 58.0 57.7 908-968 bp 1.0 82.5