Hotspot

psbZ-trnG-GCC

LSC · 38904-39425 · 522 bp

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Pi 0.0823
MarkerSeek score 85.30
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
522
variable_sites
41
pi
0.0823
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCAGATGGTTGGTCG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.0 57.8 672-676 bp 1.0 86.2
psbZ-trnG-GCC_p2 TTGCTTCTCCAGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 675-679 bp 1.0 84.7
psbZ-trnG-GCC_p3 TCCAGATGGTTGGTCGAGTA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 668-672 bp 1.0 84.6
psbZ-trnG-GCC_p4 GCTTCTCCAGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 673-677 bp 1.0 84.3
psbZ-trnG-GCC_p5 CTTCTCCAGATGGTTGGTCG TTACTTTACCCTGGGCGGAT 55.0 50.0 58.0 58.4 709-713 bp 1.0 83.9