Hotspot

trnS-UGA-psbZ

LSC · 38349-38714 · 366 bp

Back to Melia

Pi 0.00833
MarkerSeek score 45.50
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
366
variable_sites
3
pi
0.00833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 TGTCTCTCCGAGAAGTTGGT CGACCAACCATCTGGAGAAG 50.0 55.0 58.0 58.0 974-1004 bp 1.0 81.5
trnS-UGA-psbZ_p2 CAAGACCGGAGCTATCAACC CGACCAACCATCTGGAGAAG 55.0 55.0 58.1 58.0 504-522 bp 1.0 81.0
trnS-UGA-psbZ_p3 TGTCTCTCCGAGAAGTTGGT AAAAATTCGTGGGAGGGGTC 50.0 50.0 58.0 58.1 1111-1141 bp 1.0 80.9
trnS-UGA-psbZ_p4 TTTTTCTGGCTCGGCTATCC CGACCAACCATCTGGAGAAG 50.0 55.0 58.0 58.0 663 bp 0.5 59.6
trnS-UGA-psbZ_p5 TGTCTCTCCGAGAAGTTGGT CACTCTTCAGTGCGATTCCA 50.0 50.0 58.0 57.9 1532 bp 0.5 59.3