Hotspot

psbC-trnS-UGA

LSC · 38009-38255 · 247 bp

Back to Melia

Pi 0.0366
MarkerSeek score 73.90
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
247
variable_sites
9
pi
0.0366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TGATCGTGAGCTTGAACCTG GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.9 58.1 370-382 bp 1.0 81.0
psbC-trnS-UGA_p2 GATCGTGAGCTTGAACCTGT GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.9 58.1 369-381 bp 1.0 81.0
psbC-trnS-UGA_p3 CGTGAGCTTGAACCTGTTCT GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.1 58.1 366-378 bp 1.0 80.6
psbC-trnS-UGA_p4 TCGTGAGCTTGAACCTGTTC GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.1 58.1 367-379 bp 1.0 80.6
psbC-trnS-UGA_p5 TGATCGTGAGCTTGAACCTG GATGGCCGAGTGGTTGATAG 50.0 55.0 57.9 58.1 381-393 bp 1.0 80.4