Hotspot

psbM-trnD-GUC

LSC · 32159-32683 · 525 bp

Back to Melia

Pi 0.021
MarkerSeek score 59.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
525
variable_sites
11
pi
0.021
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 636-640 bp 1.0 59.7
psbM-trnD-GUC_p2 TTGACTGACCGTTTTTACGT TTCAATTGGTCAGAGCACCG 40.0 50.0 55.6 58.5 685-689 bp 1.0 58.0
psbM-trnD-GUC_p3 ACTTTGACTGACCGTTTTTACG TTCAATTGGTCAGAGCACCG 40.9 50.0 57.5 58.5 688-692 bp 1.0 57.3
psbM-trnD-GUC_p4 CTTTGACTGACCGTTTTTACGT TTCAATTGGTCAGAGCACCG 40.9 50.0 57.5 58.5 687-691 bp 1.0 57.3
psbM-trnD-GUC_p5 TCACTTTGACTGACCGTTTT TTCAATTGGTCAGAGCACCG 40.0 50.0 55.4 58.5 690-694 bp 1.0 57.0