Hotspot

atpH-atpI

LSC · 15101-16319 · 1219 bp

Back to Melia

Pi 0.0159
MarkerSeek score 69.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1219
variable_sites
19
pi
0.0159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG TACAAGCGGGATTCAAGCTC 50.0 50.0 58.0 58.0 1351-1364 bp 1.0 82.2
atpH-atpI_p2 AACGGAAGCAGCAGAAATCA TACAAGCGGGATTCAAGCTC 45.0 50.0 58.1 58.0 1315-1328 bp 1.0 81.7
atpH-atpI_p3 TACCTTGACCAACTCCAGGT TACAAGCGGGATTCAAGCTC 50.0 50.0 57.9 58.0 1373-1386 bp 1.0 81.5
atpH-atpI_p4 AATAGAAGCAAGCCCGACAG TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.0 57.9 1327-1340 bp 1.0 81.4
atpH-atpI_p5 GCAGTACCTTGACCAACTCC TACAAGCGGGATTCAAGCTC 55.0 50.0 58.2 58.0 1377-1390 bp 1.0 80.9