Hotspot

ycf1

SSC · 126207-131762 · 5556 bp

Back to Melastoma

Pi 0.00186

MarkerSeek score 34.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5556
variable_sites: 28
pi: 0.00186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAGCTGCAGAATCTTCTTC	ATGAACCCCTGGATGATCCC	50.0	55.0	57.2	58.8	281 bp	1.0	68.6
ycf1_p2	GCAGCTGCAGAATCTTCTTC	AATGAACCCCTGGATGATCC	50.0	50.0	57.2	56.9	282 bp	1.0	66.9
ycf1_p3	GCAGCTGCAGAATCTTCTTC	AAATGAACCCCTGGATGATCC	50.0	47.6	57.2	57.7	283 bp	1.0	64.8
ycf1_p4	GCAGCTGCAGAATCTTCTTC	GAACCCCTGGATGATCCCA	50.0	57.9	57.2	58.4	279 bp	1.0	64.4
ycf1_p5	GCAGCTGCAGAATCTTCTTC	TGAACCCCTGGATGATCCC	50.0	57.9	57.2	58.4	280 bp	1.0	64.4