Hotspot

ndhE

SSC · 120100-120405 · 306 bp

Back to Melastoma

Pi 0

MarkerSeek score 20.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GCCTTACATCCATCCCAAGG	TTTGGACTGTTGGGAATGGG	55.0	50.0	58.0	58.0	1134-1143 bp	1.0	82.0
ndhE_p2	ACCCATGCTTCGAGTTGTTT	TTTGGACTGTTGGGAATGGG	45.0	50.0	58.0	58.0	1261-1270 bp	1.0	81.7
ndhE_p3	GGACAAGCGGATTCACATCT	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1203-1212 bp	1.0	81.7
ndhE_p4	GCTTCGAGTTGTTTCATGCC	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1255-1264 bp	1.0	81.7
ndhE_p5	ACGGGTTTTGTGTCGGTAAA	TTTGGACTGTTGGGAATGGG	45.0	50.0	58.0	58.0	1371-1380 bp	1.0	81.5