Hotspot

clpP

LSC · 71644-73827 · 2184 bp

Back to Melastoma

Pi 0.00275

MarkerSeek score 42.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2184
variable_sites: 13
pi: 0.00275
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AACTTATTCCCTCTATTTGCT	TGCATTGTTACGCTTCCACA	33.3	45.0	52.2	58.4	2260-2273 bp	1.0	41.1
clpP_p2	ACTTATTCCCTCTATTTGCTT	TGCATTGTTACGCTTCCACA	33.3	45.0	52.2	58.4	2259-2272 bp	1.0	41.1
clpP_p3	AAACTTATTCCCTCTATTTGCT	TGCATTGTTACGCTTCCACA	31.8	45.0	53.1	58.4	2261-2274 bp	1.0	41.1
clpP_p4	AACTTATTCCCTCTATTTGCTT	TGCATTGTTACGCTTCCACA	31.8	45.0	53.1	58.4	2260-2273 bp	1.0	41.1
clpP_p5	ACTTATTCCCTCTATTTGCTTT	TGCATTGTTACGCTTCCACA	31.8	45.0	53.1	58.4	2259-2272 bp	1.0	41.1