Hotspot

ndhE-ndhG

SSC · 120406-120646 · 241 bp

Back to Melastoma

Pi 0.00968

MarkerSeek score 59.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 241
variable_sites: 6
pi: 0.00968
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCAGACACATAAGAGCCCT	AATTGCTGTTGCCCGTCAAT	50.0	45.0	57.7	59.0	358-367 bp	1.0	75.0
ndhE-ndhG_p2	TCCAGACACATAAGAGCCCT	TGCTTTGATAGGTGCAATTGC	50.0	42.9	57.7	57.8	373-382 bp	1.0	73.4
ndhE-ndhG_p3	TCCAGACACATAAGAGCCCT	ATTGCTGTTGCCCGTCAAT	50.0	47.4	57.7	58.3	357-366 bp	1.0	72.6
ndhE-ndhG_p4	TCCAGACACATAAGAGCCCT	GCTTTGATAGGTGCAATTGCT	50.0	42.9	57.7	57.5	372-381 bp	1.0	71.1
ndhE-ndhG_p5	TCCAGACACATAAGAGCCCT	AATTGCTGTTGCCCGTCAA	50.0	47.4	57.7	58.6	358-367 bp	1.0	70.7