Hotspot

psbB-psbT

LSC · 75756-75949 · 194 bp

Back to Melastoma

Pi 0.00515

MarkerSeek score 48.70

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 3
pi: 0.00515
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTGGTATTGACCCGGATTT	TTTTCGTTGGAACTTTCGGC	50.0	45.0	57.9	57.5	357-381 bp	1.0	80.4
psbB-psbT_p2	CCAACGACAAGAAGACAGGT	TTTTCGTTGGAACTTTCGGC	50.0	45.0	57.8	57.5	294-318 bp	1.0	79.0
psbB-psbT_p3	CCGGATTTGGATGCTCAAGT	TTTTCGTTGGAACTTTCGGC	50.0	45.0	58.2	57.5	345-369 bp	1.0	78.9
psbB-psbT_p4	CGACAAGAAGACAGGTCGTC	TTTTCGTTGGAACTTTCGGC	55.0	45.0	58.3	57.5	290-314 bp	1.0	78.4
psbB-psbT_p5	TGGTATTGACCCGGATTTGG	TTTTCGTTGGAACTTTCGGC	50.0	45.0	57.6	57.5	355-379 bp	1.0	77.4