Hotspot

trnP-UGG-psaJ

LSC · 68020-68502 · 483 bp

Back to Melastoma

Pi 0.00207

MarkerSeek score 52.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 3
pi: 0.00207
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TTGTACCCAAAACAAACGCG	TCAACAGACCTGCCAAAGAC	45.0	50.0	57.8	58.0	602-606 bp	1.0	79.0
trnP-UGG-psaJ_p2	TAACGGGTAGGGATGACAGG	TCAACAGACCTGCCAAAGAC	55.0	50.0	58.2	58.0	640-644 bp	1.0	78.8
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	CAAAGACCCGAACCAGAGAG	45.0	55.0	57.8	57.9	589-593 bp	1.0	78.6
trnP-UGG-psaJ_p4	TTGTACCCAAAACAAACGCG	GTGCCACGGAAAGATAGGTT	45.0	50.0	57.8	57.9	557-561 bp	1.0	78.5
trnP-UGG-psaJ_p5	TAACGGGTAGGGATGACAGG	CAAAGACCCGAACCAGAGAG	55.0	55.0	58.2	57.9	627-631 bp	1.0	78.3