Hotspot

psaA-ycf3

LSC · 43479-44084 · 606 bp

Back to Melastoma

Pi 0.00484

MarkerSeek score 43.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 8
pi: 0.00484
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TCACAAGGCGCTTCGAATAA	50.0	45.0	57.9	57.9	702-706 bp	1.0	79.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACAAGGCGCTT	50.0	45.0	57.9	58.1	709-713 bp	1.0	79.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCACAAGGCGCTTCGAATA	50.0	45.0	57.9	57.7	703-707 bp	1.0	78.0
psaA-ycf3_p4	TTTGGCCCATTCCTCGAAAG	TCACAAGGCGCTTCGAATAA	50.0	45.0	58.5	57.9	706-710 bp	1.0	77.0
psaA-ycf3_p5	TTTGGCCCATTCCTCGAAAG	TTGAAGATCACAAGGCGCTT	50.0	45.0	58.5	58.1	713-717 bp	1.0	76.9