Hotspot

psbZ-trnG-UCC

LSC · 37529-38119 · 591 bp

Back to Melastoma

Pi 0.0079
MarkerSeek score 51.90
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
591
variable_sites
14
pi
0.0079
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 GACGGCTGGTCAAGTAACAA AATCGAACCCGCATCTTCTC 50.0 50.0 58.1 58.1 748-756 bp 1.0 80.0
psbZ-trnG-UCC_p2 GACGGCTGGTCAAGTAACAA GAATCGAACCCGCATCTTCT 50.0 50.0 58.1 58.1 749-757 bp 1.0 80.0
psbZ-trnG-UCC_p3 CCCGTTGTTTTTGCTTCTCC AATCGAACCCGCATCTTCTC 50.0 50.0 57.9 58.1 769-777 bp 1.0 79.9
psbZ-trnG-UCC_p4 CCCGTTGTTTTTGCTTCTCC GAATCGAACCCGCATCTTCT 50.0 50.0 57.9 58.1 770-778 bp 1.0 79.9
psbZ-trnG-UCC_p5 GACGGCTGGTCAAGTAACAA ACTTTAACTTAACCCGGGCG 50.0 50.0 58.1 58.2 777-785 bp 1.0 78.9