Hotspot

trnD-GUC-trnY-GUA

LSC · 31244-31699 · 456 bp

Back to Melastoma

Pi 0.0038

MarkerSeek score 52.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 5
pi: 0.0038
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	517-528 bp	1.0	77.8
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	516-527 bp	1.0	77.8
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	538-549 bp	1.0	76.7
trnD-GUC-trnY-GUA_p4	TTTATTCCGTCGGGACTGAC	TGGCAATATGTCTACGCTGG	50.0	50.0	57.3	57.8	570-581 bp	1.0	76.6
trnD-GUC-trnY-GUA_p5	TTTATTCCGTCGGGACTGAC	GGCAATATGTCTACGCTGGT	50.0	50.0	57.3	57.8	569-580 bp	1.0	76.5