Hotspot

trnS-GCU-trnR-UCU

LSC · 8191-10029 · 1839 bp

Back to Melastoma

Pi 0.00163

MarkerSeek score 53.70

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1839
variable_sites: 8
pi: 0.00163
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	CGAACTTGCGTCCAATAGGA	50.0	50.0	58.0	58.0	1944-1952 bp	1.0	80.6
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	CGAACTTGCGTCCAATAGGA	50.0	50.0	58.0	58.0	1943-1951 bp	1.0	80.6
trnS-GCU-trnR-UCU_p3	ACGGAAAGAGAGGGATTCGA	CGAACTTGCGTCCAATAGGA	50.0	50.0	58.2	58.0	1998-2006 bp	1.0	79.4
trnS-GCU-trnR-UCU_p4	CAATCCGACGCTTTAGTCCA	ACGAACTTGCGTCCAATAGG	50.0	50.0	58.0	58.3	1945-1953 bp	1.0	78.4
trnS-GCU-trnR-UCU_p5	AATCCGACGCTTTAGTCCAC	ACGAACTTGCGTCCAATAGG	50.0	50.0	58.0	58.3	1944-1952 bp	1.0	78.4