Hotspot

rps16-trnQ-UUG

LSC · 5802-6918 · 1117 bp

Back to Melastoma

Pi 0.00531

MarkerSeek score 55.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 17
pi: 0.00531
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGCCACACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1154-1190 bp	1.0	82.8
rps16-trnQ-UUG_p2	ATCCACGAACGATCCTCTCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1177-1213 bp	1.0	82.5
rps16-trnQ-UUG_p3	GAACGATCCTCTCTGGCAAG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	1171-1207 bp	1.0	82.5
rps16-trnQ-UUG_p4	ACACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.7	58.1	1149-1185 bp	1.0	80.5
rps16-trnQ-UUG_p5	AAGCCACACGTTGCTTTCTA	AACAGACTGACAAATGGGGC	45.0	50.0	58.0	58.4	1216-1252 bp	1.0	80.2