Hotspot

orf120

Genome · 202043-202405 · 363 bp

Back to Melanthalia

Pi 0.492

MarkerSeek score 76.50

Primer available yes

Label orf120

Feature Metadata

label_name: orf120
feature_type: gene
region: Genome
length_bp: 363
variable_sites: 156
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf120_p1	TGGCAACTTCAGCTTTCTGT	AAGCACCCAGAGACAAGTTG	45.0	50.0	57.9	58.0	724 bp	0.5	59.3
orf120_p2	TGGCAACTTCAGCTTTCTGT	TTTTTCGAGGTGAGTGGCAA	45.0	45.0	57.9	58.0	1277 bp	0.5	59.2
orf120_p3	TCGCCACAGAATGCACTTTA	AAGCACCCAGAGACAAGTTG	45.0	50.0	57.8	58.0	636 bp	0.5	58.4
orf120_p4	TCGCCACAGAATGCACTTTA	TTTTTCGAGGTGAGTGGCAA	45.0	45.0	57.8	58.0	1189 bp	0.5	58.2
orf120_p5	TGGCAACTTCAGCTTTCTGT	TTTTCGAGGTGAGTGGCAAT	45.0	45.0	57.9	57.7	1276 bp	0.5	57.4