Hotspot

orf88-orf89

Genome · 199498-200737 · 1240 bp

Back to Melanthalia

Pi 0.458

MarkerSeek score 77.10

Primer available yes

Label orf88-orf89

Feature Metadata

label_name: orf88-orf89
feature_type: igs
region: Genome
length_bp: 1240
variable_sites: 407
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf88-orf89_p1	ATAGCATCCAGAGGCTCGTA	ACCTTTTTCATCCTGCTGCA	50.0	45.0	57.7	58.0	1730 bp	0.5	57.6
orf88-orf89_p2	ATAGCATCCAGAGGCTCGTA	TGCACCTTTTTCATCCTGCT	50.0	45.0	57.7	58.0	1733 bp	0.5	57.6
orf88-orf89_p3	ACGTCTTTCCGTTTGGACTT	ACCTTTTTCATCCTGCTGCA	45.0	45.0	57.7	58.0	1787 bp	0.5	57.4
orf88-orf89_p4	ACGTCTTTCCGTTTGGACTT	TGCACCTTTTTCATCCTGCT	45.0	45.0	57.7	58.0	1790 bp	0.5	57.4
orf88-orf89_p5	ATAGCATCCAGAGGCTCGTA	GTGCACCTTTTTCATCCTGC	50.0	50.0	57.7	57.9	1734 bp	0.5	57.1