Hotspot

psbD-ompR

Genome · 192724-193622 · 899 bp

Back to Melanthalia

Pi 0.183

MarkerSeek score 83.40

Primer available yes

Label psbD-ompR

Feature Metadata

label_name: psbD-ompR
feature_type: igs
region: Genome
length_bp: 899
variable_sites: 152
pi: 0.183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-ompR_p1	TGGACCCCACAGCAGTAATA	GCAAGAAGTTTGGGGGTACA	50.0	50.0	58.0	58.0	1353 bp	0.5	59.6
psbD-ompR_p2	TGGACCCCACAGCAGTAATA	TGCAAGAAGTTTGGGGGTAC	50.0	50.0	58.0	58.0	1354 bp	0.5	59.6
psbD-ompR_p3	CGCTAAGCCGTGAGTATACC	GCAAGAAGTTTGGGGGTACA	55.0	50.0	58.0	58.0	1260 bp	0.5	59.5
psbD-ompR_p4	CGCTAAGCCGTGAGTATACC	TGCAAGAAGTTTGGGGGTAC	55.0	50.0	58.0	58.0	1261 bp	0.5	59.5
psbD-ompR_p5	CCCATGCTATTTGCTGGAGT	GCAAGAAGTTTGGGGGTACA	50.0	50.0	57.9	58.0	1325 bp	0.5	59.4