Hotspot

clpC-psbW

Genome · 166870-173228 · 6359 bp

Back to Melanthalia

Pi 0.345
MarkerSeek score 68.90
Primer available yes
Label clpC-psbW

Feature Metadata

label_name
clpC-psbW
feature_type
igs
region
Genome
length_bp
6359
variable_sites
1256
pi
0.345
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.57
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpC-psbW_p1 TGCGCAAAAAGTAGGTTCAG GCTTGCAGGGCTTTCATGTA 45.0 50.0 56.9 58.8 707 bp 0.5 45.0
clpC-psbW_p2 GCGCAAAAAGTAGGTTCAGT GCTTGCAGGGCTTTCATGTA 45.0 50.0 56.9 58.8 706 bp 0.5 44.9
clpC-psbW_p3 TGCGCAAAAAGTAGGTTCAG GCACAGCTTAACACTACTGC 45.0 50.0 56.9 57.1 850 bp 0.5 44.5
clpC-psbW_p4 GCGCAAAAAGTAGGTTCAGT GCACAGCTTAACACTACTGC 45.0 50.0 56.9 57.1 849 bp 0.5 44.5
clpC-psbW_p5 TTGCGCAAAAAGTAGGTTCAG GCTTGCAGGGCTTTCATGTA 42.9 50.0 57.7 58.8 708 bp 0.5 42.6