Hotspot

trnR(ccg)-orf460

Genome · 63449-65038 · 1590 bp

Back to Melanthalia

Pi 0.208

MarkerSeek score 81.90

Primer available yes

Label trnR(ccg)-orf460

Feature Metadata

label_name: trnR(ccg)-orf460
feature_type: igs
region: Genome
length_bp: 1590
variable_sites: 320
pi: 0.208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ccg)-orf460_p1	GTTTCCTCCTGGCCGTAAAA	ACAATGGAAAAGGATGCCGA	50.0	45.0	58.1	57.8	2795 bp	0.5	57.5
trnR(ccg)-orf460_p2	GCCTAAAGGTTCAGTCAGCA	ACAATGGAAAAGGATGCCGA	50.0	45.0	57.8	57.8	2497 bp	0.5	56.8
trnR(ccg)-orf460_p3	TGCCTAAAGGTTCAGTCAGC	ACAATGGAAAAGGATGCCGA	50.0	45.0	57.8	57.8	2498 bp	0.5	56.8
trnR(ccg)-orf460_p4	TTTCCTCCTGGCCGTAAAAG	ACAATGGAAAAGGATGCCGA	50.0	45.0	57.8	57.8	2794 bp	0.5	56.7
trnR(ccg)-orf460_p5	AGAGCGTGTAACTCAGAGGA	ACAATGGAAAAGGATGCCGA	50.0	45.0	57.8	57.8	2178 bp	0.5	56.7