Hotspot

psaC-ycf22

Genome · 54544-55261 · 718 bp

Back to Melanthalia

Pi 0.152

MarkerSeek score 83.20

Primer available yes

Label psaC-ycf22

Feature Metadata

label_name: psaC-ycf22
feature_type: igs
region: Genome
length_bp: 718
variable_sites: 102
pi: 0.152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ycf22_p1	AGCAGGTATGACAGGTGGAT	ATTCCAGCTCGAATATCGGC	50.0	50.0	58.1	57.9	2037 bp	0.5	58.4
psaC-ycf22_p2	CCCCTGGGATGGTTGTAAAG	ATTCCAGCTCGAATATCGGC	55.0	50.0	57.9	57.9	1450 bp	0.5	58.2
psaC-ycf22_p3	ACTAGTCGAAGCATGGGTCT	ATTCCAGCTCGAATATCGGC	50.0	50.0	58.1	57.9	1320 bp	0.5	58.1
psaC-ycf22_p4	GGGATGGTTGTAAAGCTGCT	ATTCCAGCTCGAATATCGGC	50.0	50.0	58.2	57.9	1445 bp	0.5	58.0
psaC-ycf22_p5	TGCTCCTAGAACCGAGGATT	ATTCCAGCTCGAATATCGGC	50.0	50.0	57.8	57.9	1414 bp	0.5	58.0