Hotspot

ycf21-cpcA

Genome · 46096-47748 · 1653 bp

Back to Melanthalia

Pi 0.257

MarkerSeek score 80.70

Primer available yes

Label ycf21-cpcA

Feature Metadata

label_name: ycf21-cpcA
feature_type: igs
region: Genome
length_bp: 1653
variable_sites: 415
pi: 0.257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf21-cpcA_p1	TAGCCTCCATTCTGCAGGAA	GAGCATCAGTAGCAGTTGCT	50.0	50.0	58.4	58.0	2458 bp	0.5	56.5
ycf21-cpcA_p2	TTAGCCTCCATTCTGCAGGA	GAGCATCAGTAGCAGTTGCT	50.0	50.0	58.4	58.0	2459 bp	0.5	56.5
ycf21-cpcA_p3	TAGCCTCCATTCTGCAGGAA	CATGGACTAGGTGGACAAGC	50.0	55.0	58.4	58.0	1836 bp	0.5	56.4
ycf21-cpcA_p4	TTAGCCTCCATTCTGCAGGA	CATGGACTAGGTGGACAAGC	50.0	55.0	58.4	58.0	1837 bp	0.5	56.4
ycf21-cpcA_p5	TAGCCTCCATTCTGCAGGAA	ATGGACTAGGTGGACAAGCT	50.0	50.0	58.4	58.0	1835 bp	0.5	56.3