Hotspot

rps16

LSC · 5118-6231 · 1114 bp

Back to Melanoseris

Pi 0.0027

MarkerSeek score 60.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1114
variable_sites: 7
pi: 0.0027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAAATCACTATCCTACCCTTAGT	TCTCTGTACCGACTGAACCA	37.5	50.0	56.1	57.7	1234-1260 bp	1.0	41.3
rps16_p2	AGAAATCACTATCCTACCCT	TCTCTGTACCGACTGAACCA	40.0	50.0	52.0	57.7	1234-1260 bp	1.0	41.3
rps16_p3	AGAGCAAGAAAAGAAGGAAA	TCTCTGTACCGACTGAACCA	35.0	50.0	52.6	57.7	1293-1319 bp	0.8	33.1
rps16_p4	AGAGCAAGAAAAGAAGGAAAT	TCTCTGTACCGACTGAACCA	33.3	50.0	53.3	57.7	1293-1319 bp	0.8	33.1
rps16_p5	AGAGCAAGAAAAGAAGGAAA	GTCTCTGTACCGACTGAACC	35.0	55.0	52.6	57.4	1294-1320 bp	0.8	33.1