Hotspot

rpoC2

LSC · 18971-23116 · 4146 bp

Back to Melanoseris

Pi 0.000531

MarkerSeek score 54.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 5
pi: 0.000531
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TAGCGGCTTCAAAACACCTT	TAACGGGACCAACAGATCCT	45.0	50.0	58.0	58.1	252 bp	1.0	79.7
rpoC2_p2	GTTGGTAGCGGCTTCAAAAC	TAACGGGACCAACAGATCCT	50.0	50.0	58.0	58.1	257 bp	1.0	79.5
rpoC2_p3	AAACACCTTCAAGCGAACCT	TAACGGGACCAACAGATCCT	45.0	50.0	57.9	58.1	241 bp	1.0	79.5
rpoC2_p4	AGAAAACGTTGTTCTGGGGG	TAACGGGACCAACAGATCCT	50.0	50.0	58.3	58.1	288 bp	1.0	77.4
rpoC2_p5	TTGGTAGCGGCTTCAAAACA	TAACGGGACCAACAGATCCT	45.0	50.0	58.3	58.1	256 bp	1.0	77.3