Hotspot

rpoC1

LSC · 16072-18869 · 2798 bp

Back to Melanoseris

Pi 0.000715

MarkerSeek score 56.80

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2798
variable_sites: 5
pi: 0.000715
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GTTCGTTCGAGAACTACGATCT	ACAATGGGCTTGAGTCAGTG	45.5	50.0	58.0	58.1	2993-2997 bp	1.0	63.4
rpoC1_p2	GTTCGTTCGAGAACTACGATCT	CAATGGGCTTGAGTCAGTGA	45.5	50.0	58.0	57.8	2992-2996 bp	1.0	62.7
rpoC1_p3	GTTCGTTCGAGAACTACGATCT	TGACAATGGGCTTGAGTCAG	45.5	50.0	58.0	57.8	2995-2999 bp	1.0	62.7
rpoC1_p4	GTTCGTTCGAGAACTACGATCT	TGGGCTTGAGTCAGTGATTG	45.5	50.0	58.0	57.8	2989-2993 bp	1.0	62.7
rpoC1_p5	GTTCGTTCGAGAACTACGATCT	GACAATGGGCTTGAGTCAGT	45.5	50.0	58.0	57.8	2994-2998 bp	1.0	62.7