Hotspot

matK

LSC · 2064-3584 · 1521 bp

Back to Melanoseris

Pi 0.00184

MarkerSeek score 52.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 6
pi: 0.00184
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ATTTACAAGGTTTCATAACGAA	ACCCCCAAAATCCTCTACCT	27.3	50.0	52.1	57.6	1584 bp	1.0	40.3
matK_p2	ATTTACAAGGTTTCATAACGAA	AACCCCCAAAATCCTCTACC	27.3	50.0	52.1	56.8	1585 bp	1.0	40.3
matK_p3	TATTTACAAGGTTTCATAACGAA	ACCCCCAAAATCCTCTACCT	26.1	50.0	52.2	57.6	1585 bp	1.0	40.3
matK_p4	ATTTACAAGGTTTCATAACGAA	AACCCCCAAAATCCTCTACCT	27.3	47.6	52.1	58.3	1585 bp	1.0	40.3
matK_p5	ATTTACAAGGTTTCATAACGAA	ACCCCCAAAATCCTCTACCTT	27.3	47.6	52.1	58.3	1584 bp	1.0	40.3